Minimzing the system with 25 kcal/mol restraints on DNA, 500 steps of steepest descent and 500 of conjugated gradient &cntrl imin=1, ntx=1, irest=0, ntpr=50, ntf=1, ntb=1, cut=9.0, nsnb=10, ntr=1, maxcyc=1000, ncyc=500, ntmin=1, restraintmask=':1-24', restraint_wt=25.0, &end &ewald ew_type = 0, skinnb = 1.0, &end